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技巧1：先禮后兵，禮貌開頭

Dear Editor and Reviewers,

Thank you for your letter and for the reviewers’ comments concerning our manuscript (MS No.: XXX).

These comments are all valuable and very helpful for revising and improving our paper, as well as the important guiding significance to our researches. We have studied comments carefully and have made corrections accordingly which we hope meet with your approval. The responds to the reviewer’s comments are as follows:

.......

技巧2：細(xì)節(jié)修改，不容小覷，一一作答，有禮有節(jié)

1. 比如審稿人提出：圖中需要補充比例尺

Reply: As for the referee’s concern (或As suggested), we have added scale bars to the Figures.

2. 比如審稿人提出：部分圖片沒有顯著性差異

Reply: Thank you very much for your careful reading of our paper (或We feel so sorry for our carelessness), we have indicated a significant difference with asterisk in the figures and also checked all the other figures. We confirm that all figures are correct now. (一旦被指出某一點問題，也要一并檢查這一類問題，確保所有圖片沒有同樣的情況)

3. 比如審稿人提出：某些具體的參考文獻作者名字不對，或期刊名不對，或不是英文文獻等

Reply: 套路同2—首先回復(fù)已更正了這些被指出的文獻問題，另外還檢查了其他文獻，并做了相應(yīng)更改，確認(rèn)所有文獻現(xiàn)在都正確。(一旦被指出某一點問題，也要一并檢查這一類問題，杜絕再次出現(xiàn)，也可以減輕后期proof的校對工作)

4. 比如審稿人提出：通篇語言存在問題，建議母語化潤色

Reply: We feel so sorry for the mistakes in the manuscript and inconvenience they caused in your reading. The manuscript has been thoroughly revised and edited by a native English speaker (或The language in our revised manuscript has been professionally edited and verified by a native English speaker), we hope it can meet the standard for final publication. Thank you very much for your precious comments. (這種情況只能進行母語化潤色，如果語言只是稍微有點問題，建議再仔細(xì)閱讀幾遍全文加以修改)

5. 比如審稿人提出：補充XXX縮寫詞的全稱

Reply: 套路同2—首先回復(fù)已補充了這些被指出的縮寫詞的全稱，另外再檢查文中其他縮寫詞是否也有這種情況，該標(biāo)全的標(biāo)全，確認(rèn)所有縮寫詞第一次被使用時都標(biāo)明了全稱。

6. 比如審稿人提出：裂解液成分有問題

Reply: We are very sorry for the mistake. Lentivirus-transduced cells were washed twice with PBS, and suspended in a lysis buffer (2% mercaptoethanol, 20% glycerol, and 4% SDS, in 100 mM Tris-HCl buffer, pH 6.8). The information for the lysis buffer has been corrected in the materials and methods. (先回復(fù)下正確的成分應(yīng)該是什么，并說明這些信息已在文中被同步更正過來了)

技巧3：補充實驗，耗時耗力，如果不補，只能argue

1. 比如審稿人提出：補充正常皮膚細(xì)胞作對照

Tips: 如果你真的不想補，或者也買不到正常細(xì)胞株，還是查一查正常細(xì)胞株的名字，告訴審稿人你確實努力過，只是事與愿違??！

Reply: Indeed, it will be more convincing if we get a comparative assessment on normal keratinocytes. However, the normal skin cell line TE 353.SK that we recently obtained could not be cultured stably under the condition of our lab at this time. We are also collecting human tissue samples from patients with primary melanoma and benign nevi. Based on our data, XXX expression will be analyzed in clinical samples by immunohistochemistry, and the correlation between XXX expression and the prognosis of melanoma will be determined in our subsequent investigations. Therefore, the referee’s concern is of importance for our further study, and we will show the results in our next paper for XXX.

2. 比如審稿人提出：補充體內(nèi)實驗

Tips: 補充動物實驗，是審稿人常提的問題，一點不令人意外。既然躲不過，就見招拆招。如果能補上，自然極好；如果沒有條件補，或者實驗來不及，也要盡量說的委婉點，假裝你確實努力了！

Reply: We deeply appreciative the reviewer’s suggestion. Indeed, it will be more profound if we get the relevant results in vivo.

我們?nèi)ベI了若干只小鼠，按照文獻的方法操作: We have purchased 10 thymus-null BALB/c nude mice (female, age 4-6 weeks) from the Animal Center of Chinese Academy of Medical Sciences. Osteosarcoma xenografts were established in nude mice according to a previous report (Gao et al., 2009). Briefly, control lentivirus or XXX gene shRNA-expressing lentivirus infected XXX cells (1×105) were suspended in 0.1 ml PBS and then injected subcutaneously into the proximal tibia of each anesthetized nude mice (n = 5 in each group). Unfortunately, the xenografts could not grow after inoculation for three weeks (滿懷欣喜的接種到動物身上，然而實驗這東西，哪能一遍就成呢，我們做了一遍，結(jié)果沒能成瘤，實在是悲劇了). The limitation of technical condition may be the main reason. Also, we doubt that the cell viability could be insufficient to initiate osteosarcoma tumorigenesis in vivo due to the long-distance transport.

Therefore, we seek for the editor’s tolerance and understanding. Many thanks for your kind help!

Reference: Gao et al. (2009) …………

補充實驗，究竟是申請延期，老老實實把實驗做完，還是投機取巧argue一下，要看自己的運氣了。一般情況，可以對照審稿人的意見綜合權(quán)衡。高分文章，最好還是該補的全補了，低分文章，如果你覺得有80%的把握，完全可以憑你的勁爆人品成功拿下審稿人。

技巧4：審稿人的其他一些疑慮

Tips: 審稿人對文章提出的其他一些疑惑，不是僅僅為了得到你的回復(fù)，能消除這些疑惑的statement，都應(yīng)盡可能地融合到文章里，讓它成為文章的一部分，而不是Response里寫了一大堆，正文里卻一句話沒有，畢竟最后發(fā)表的是Article，而不是Response。

Reply: 先仔細(xì)作解釋，并聲明在修改稿中已對這些意見作了相應(yīng)的補充。(注意是融合到文章中，而不是直接復(fù)制冗長的解釋到文章里。融合到文中的目的既豐富了文章內(nèi)容，也是為了在文章發(fā)表后可以消除其他讀者同樣的疑慮)

總之，所有的意見回復(fù)和修改，都是為了讓文章更好更快的接收！